6ALM
VioC L-arginine hydroxylase bound to Fe(II), L-arginine, and 2-OXO-GLUTARIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.336, 66.990, 62.665 |
Unit cell angles | 90.00, 108.81, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.600 |
R-factor | 0.1993 |
Rwork | 0.198 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wbo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.947 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.053 | 0.021 | 0.657 |
Rmeas | 0.057 | 0.023 | 0.705 |
Rpim | 0.020 | 0.008 | 0.255 |
Total number of observations | 311707 | ||
Number of reflections | 40551 | 2124 | 1907 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 97.4 | 99.5 | 92.5 |
Redundancy | 7.7 | 7.4 | 7.3 |
CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M 2-oxoglutarate, 0.1 M Tris pH 8.5 |