6ALM

VioC L-arginine hydroxylase bound to Fe(II), L-arginine, and 2-OXO-GLUTARIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.336, 66.990, 62.665
Unit cell angles	90.00, 108.81, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.1993
Rwork	0.198
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wbo
RMSD bond length	0.004
RMSD bond angle	0.947
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.053	0.021	0.657
Rmeas	0.057	0.023	0.705
Rpim	0.020	0.008	0.255
Total number of observations	311707
Number of reflections	40551	2124	1907
<I/σ(I)>	8.1
Completeness [%]	97.4	99.5	92.5
Redundancy	7.7	7.4	7.3
CC(1/2)		1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M 2-oxoglutarate, 0.1 M Tris pH 8.5