6ALD
RABBIT MUSCLE ALDOLASE A/FRUCTOSE-1,6-BISPHOSPHATE COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.800, 100.600, 84.500 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.300 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ado |
| RMSD bond length | 0.004 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 * | 0.182 * |
| Number of reflections | 62360 | |
| <I/σ(I)> | 7.2 | 2.43 |
| Completeness [%] | 94.5 | 95.3 * |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 4 * | pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.2 (M) | |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 2 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1.1 (mM) | |
| 6 | 1 | drop | PEG6000 | 10 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | PEG6000 | 24 (%) |
