6AL6

Crystal structure HpiC1 in P42 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03
Spacegroup name	P 42
Unit cell lengths	71.339, 71.339, 80.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.764 - 2.088
R-factor	0.2089
Rwork	0.206
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wpp
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.760
High resolution limit [Å]	2.090
Number of reflections	24033
<I/σ(I)>	18.94
Completeness [%]	98.9
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	22% PEG 4000, 200 mM CaCl2, 100 mM Tris pH 8.5, 5% ethylene glycol