6AKM

Crystal structure of SLMAP-SIKE1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
Unit cell lengths	66.763, 66.763, 61.353
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.080 - 2.300
R-factor	0.2074
Rwork	0.204
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6akk
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.094	0.821
Rmeas	0.097	0.844
Rpim	0.022	0.193
Number of reflections	7336	713
<I/σ(I)>	21.6	5.2
Completeness [%]	100.0	100
Redundancy	18.8	19
CC(1/2)		0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium citrate, pH 5.5; 15% (w/v) PEG 6000