6AKI
Calcium release-activated calcium channel protein 1, P288L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.107, 90.659, 178.721 |
| Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
| Resolution | 26.475 - 4.496 |
| R-factor | 0.3306 |
| Rwork | 0.325 |
| R-free | 0.38620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.660 |
| High resolution limit [Å] | 4.490 | 4.490 |
| Number of reflections | 17659 | |
| <I/σ(I)> | 7.94 | |
| Completeness [%] | 80.7 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.05M NaCl,0.02M MgCl2, 0.1M Sodium citrate pH 6.5, 16-20% PEG400 |
