6AJR

Complex form of Uracil DNA glycosylase X and uracil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	190
Detector technology	CCD
Collection date	2015-07-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	36.479, 51.773, 55.107
Unit cell angles	90.00, 104.98, 90.00

Refinement procedure

Resolution	19.008 - 1.341
R-factor	0.1397
Rwork	0.138
R-free	0.16570
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.360
High resolution limit [Å]	1.340	1.340
Rmerge	0.054	0.238
Number of reflections	43403	2162
<I/σ(I)>	54.35
Completeness [%]	97.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	2.0M ammonium citrate tribasic pH7.0, 0.1M Bis-Tris propane pH7.0