6AJQ

E52Q mutant form of Uracil DNA glycosylase X from Mycobacterium smegmatis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	190
Detector technology	CCD
Collection date	2016-10-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	36.395, 51.602, 54.436
Unit cell angles	90.00, 105.35, 90.00

Refinement procedure

Resolution	18.400 - 1.342
R-factor	0.1743
Rwork	0.173
R-free	0.19090
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	0.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.360
High resolution limit [Å]	1.340	1.340
Rmerge	0.061	0.383
Number of reflections	41987	2098
<I/σ(I)>	33.05
Completeness [%]	96.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	2.0M ammonium citrate tribasic pH7.0, 0.1M Bis-Tris propane pH7.0