6AJP
Complex form of Uracil DNA glycosylase X and deoxyuridine monophosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2016-11-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.436, 51.209, 54.533 |
| Unit cell angles | 90.00, 104.98, 90.00 |
Refinement procedure
| Resolution | 18.877 - 1.334 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18730 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.057 | 0.153 |
| Number of reflections | 43215 | 1511 |
| <I/σ(I)> | 47.76 | |
| Completeness [%] | 96.9 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2.0M ammonium citrate tribasic pH7.0, 0.1M Bis-Tris propane pH7.0 |
