6AJO

Complex form of Uracil DNA glycosylase X and uracil-DNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	190
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	36.829, 64.450, 82.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.976 - 2.269
R-factor	0.1985
Rwork	0.192
R-free	0.25710
Structure solution method	MIR
RMSD bond length	0.021
RMSD bond angle	2.091
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.269	2.280
Rmerge	0.098	0.938
Number of reflections	9584	415
<I/σ(I)>	31.2
Completeness [%]	99.0
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.4M sodium malonate pH 7.0, 0.1 M Bis-Tris propane pH 7.0.