6AIS

Loop deletion mutant (deleting two residues)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	33.003, 54.876, 66.190
Unit cell angles	90.00, 100.54, 90.00

Refinement procedure

Resolution	19.782 - 1.300
R-factor	0.1776
Rwork	0.176
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g8c
RMSD bond length	0.005
RMSD bond angle	0.793
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.148	0.444
Number of reflections	57159	2864
<I/σ(I)>	15.1	2.2
Completeness [%]	99.7	98.7
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	292	45% w/v PEG400, 0.1 M Imidazole