6AI9
Cab2 mutant-H337A complex with phosphopantothenate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.704, 113.517, 55.777 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 56.760 - 2.090 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.760 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.087 | 0.383 |
| Rmeas | 0.104 | |
| Rpim | 0.056 | 0.056 |
| Number of reflections | 36076 | 1819 |
| <I/σ(I)> | 11.41 | 2.56 |
| Completeness [%] | 97.2 | 96.8 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 200mM ammonium tartrate, 20% PEG 3350 |
