6AI8
Cab2 mutant-H337A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.786, 113.235, 57.782 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 57.680 - 2.300 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.685 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.077 | 0.411 |
| Rmeas | 0.085 | 0.456 |
| Rpim | 0.036 | 0.196 |
| Number of reflections | 30686 | 1547 |
| <I/σ(I)> | 16.26 | 3.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.5 | 5.2 |
| CC(1/2) | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 100mM HEPES pH7.0, 18% PEG 4000 and 150mM (NH4)2SO4 |
