6AI3
Structure of the 328-692 fragment of FlhA (T490M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 41 |
| Unit cell lengths | 215.930, 215.930, 64.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.983 - 3.300 |
| R-factor | 0.2069 |
| Rwork | 0.201 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a5i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.320 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.300 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.108 | 0.461 |
| Number of reflections | 22917 | 4694 |
| <I/σ(I)> | 8.2 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES NaOH pH 7.5, 27% (w/v) PEG 400, 0.23M NaCl |
