6AHX
Copper-Sensing Operon Regulator Protein (CsoRGz)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-06 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 44.730, 44.730, 82.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.740 - 2.000 |
| R-factor | 0.19996 |
| Rwork | 0.197 |
| R-free | 0.25796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aai |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.961 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.740 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.031 | 0.028 |
| Number of reflections | 6882 | 664 |
| <I/σ(I)> | 45.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.95 | 5% (v/v) Tacsimate TM pH 7.0, 0.1 M HEPES pH 7.0, 10% (w/v) PEG monomethyl ether 5000 |
