6AHW
Crystal structure of circular-permutated YibK methyltransferase from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.716, 46.941, 55.703 |
| Unit cell angles | 90.00, 97.45, 90.00 |
Refinement procedure
| Resolution | 29.850 - 1.560 |
| R-factor | 0.19526 |
| Rwork | 0.193 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 48195 | 4806 |
| <I/σ(I)> | 23.47 | 3.88 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG8000, 0.1M sodium cacodylate, 0.2M sodium acetate, pH 6.5 |
