6AHV

Crystal structure of human RPP40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	73.398, 129.892, 116.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.434 - 2.600
R-factor	0.2198
Rwork	0.218
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.604
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.093	0.046	0.696
Rmeas	0.103	0.052	0.770
Rpim	0.045	0.023	0.323
Total number of observations	95185
Number of reflections	17458	1833	1710
<I/σ(I)>	7
Completeness [%]	99.5	98	99.9
Redundancy	5.5	5.1	5.6
CC(1/2)		0.996	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	5% v/v TacsimateTM pH 7.0, 100mM Hepes pH 7.0, 10% w/v polyethylene glycol monomethyl ether 5000