6AHQ
Structure of the 40-167 fragment of FliL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 162.139, 162.139, 302.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.551 - 3.398 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ahp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.452 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.300 | 3.500 |
| High resolution limit [Å] | 3.398 | 3.398 |
| Rmerge | 0.137 | 0.325 |
| Number of reflections | 54933 | 4467 |
| <I/σ(I)> | 10.8 | 5 |
| Completeness [%] | 98.1 | 98.8 |
| Redundancy | 7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium acetate pH 6.0, 25 % (w/v) PEG-200, 0.15M CaCl2, 1.4% (v/v) EtOH |
