6AGI
Crystal Structure of EFHA2 in Ca-binding State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9681 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.387, 77.177, 172.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.700 - 2.799 |
| R-factor | 0.2025 |
| Rwork | 0.197 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6agj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2666: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.799 | 2.799 |
| Number of reflections | 22892 | 2218 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 20 mM CHES pH 9.5, 200 mM NaCl, 2 mM CaCl2, 2 mM DTT |
