6AGI

Crystal Structure of EFHA2 in Ca-binding State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9681
Spacegroup name	P 21 21 21
Unit cell lengths	67.387, 77.177, 172.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 2.799
R-factor	0.2025
Rwork	0.197
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6agj
RMSD bond length	0.010
RMSD bond angle	1.418
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2666: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.799	2.799
Number of reflections	22892	2218
<I/σ(I)>	14.1
Completeness [%]	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	20 mM CHES pH 9.5, 200 mM NaCl, 2 mM CaCl2, 2 mM DTT