6AFB
DJ-1 C106S incubated with isatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.860, 74.860, 74.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.400 - 1.600 |
R-factor | 0.14588 |
Rwork | 0.145 |
R-free | 0.17192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1soa |
RMSD bond length | 0.018 |
RMSD bond angle | 2.026 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.400 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.091 | 0.617 |
Number of reflections | 32338 | |
<I/σ(I)> | 10.2 | 2.4 |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 100mM TRIS-HCl, 200mM sodium citrate, 30% PEG-400, 5mM DTT |