6AEQ
Crystal structure of the ssDNA-binding domain of DnaT from Salmonella enterica Serovar Typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2018-05-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.909, 92.319, 69.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.483 - 2.252 |
| R-factor | 0.222361580082 |
| Rwork | 0.220 |
| R-free | 0.26518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ou6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.052 | 0.288 |
| Number of reflections | 8294 | 764 |
| <I/σ(I)> | 30.14 | 4.58 |
| Completeness [%] | 98.4 | 91.8 |
| Redundancy | 5.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M Magnesium Sulfate, 100mM MES Sodium Salt pH 6.5 |
