6AEF
PapA2 acyl transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97883 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.707, 100.668, 128.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.714 - 2.160 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23150 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.711 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.714 | 2.250 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 52880 | |
| <I/σ(I)> | 2.24 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | PEG 8000, magnesium chloride, sodium cacodylate |
