6AED
Crystal Structure of the four Ig-like domain of LILRB2(LIR2/ILT4/CD85d)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 61 |
| Unit cell lengths | 172.196, 172.196, 65.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.752 - 3.797 |
| R-factor | 0.2651 |
| Rwork | 0.264 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gw5 4lla |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.797 | 3.800 |
| Number of reflections | 11011 | 1114 |
| <I/σ(I)> | 16.83 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris, pH 8.5, 25%(v/v) tert-butanol |
