6AED

Crystal Structure of the four Ig-like domain of LILRB2(LIR2/ILT4/CD85d)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 61
Unit cell lengths	172.196, 172.196, 65.602
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.752 - 3.797
R-factor	0.2651
Rwork	0.264
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gw5 4lla
RMSD bond length	0.003
RMSD bond angle	0.702
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.940
High resolution limit [Å]	3.797	3.800
Number of reflections	11011	1114
<I/σ(I)>	16.83
Completeness [%]	99.9
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Tris, pH 8.5, 25%(v/v) tert-butanol