6AE4
Crystals structure of Classical swine fever virus NS5B (residues 1-694, Y471A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 161.492, 161.492, 56.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.037 - 2.950 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cjq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.004 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
Rmerge | 0.148 | 0.079 | 0.518 |
Rmeas | 0.155 | 0.084 | 0.542 |
Rpim | 0.047 | 0.027 | 0.159 |
Number of reflections | 16243 | 1761 | 1577 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 11.3 | 10 | 11.5 |
CC(1/2) | 0.997 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Polypropylene glycol 400 |