6AE1

Crystal structure of Csm2 of the type III-A CRISPR-Cas effector complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2014-12-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97875
Spacegroup name	C 1 2 1
Unit cell lengths	98.496, 67.503, 69.638
Unit cell angles	90.00, 134.92, 90.00

Refinement procedure

Resolution	49.308 - 2.405
R-factor	0.213
Rwork	0.212
R-free	0.23300
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.080	0.057	0.505
Rmeas	0.087	0.062	0.556
Rpim	0.033	0.024	0.230
Total number of observations	84040
Number of reflections	12646	660	606
<I/σ(I)>	5.5
Completeness [%]	100.0	100	99.8
Redundancy	6.6	6.4	5.6
CC(1/2)		0.997	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	180 mM Li2SO4, 90 mM HEPES-Na (pH 7.5), 22.5% (w/v) PEG3350, 10 mM sarcosine