6ADZ
LdCoroCC Double mutant- I486A-L493A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 67.877, 67.877, 208.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.316 - 2.431 |
| R-factor | 0.2807 |
| Rwork | 0.277 |
| R-free | 0.31430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ado |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 (V703g) |
| Data scaling software | HKL-2000 (V703g) |
| Phasing software | PHASER (1.8.4_1496) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.320 | 2.517 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.059 | 0.860 |
| Rmeas | 0.061 | |
| Number of reflections | 11408 | 1056 |
| <I/σ(I)> | 24.96 | 2.33 |
| Completeness [%] | 99.7 | 96.91 |
| Redundancy | 16.1 | 13.8 |
| CC(1/2) | 0.999 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 297 | Lithium sulfate, Ammonium sulfate and Sodium citrate 5.6 |
