6ADO
LdCoroCC mutant-I486A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.338, 53.338, 142.949 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.977 - 2.502 |
| R-factor | 0.2696 |
| Rwork | 0.268 |
| R-free | 0.29450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-2000 (v703g) |
| Data scaling software | HKL-2000 (v703g) |
| Phasing software | PHASER (V1.8.4.1496) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.980 | 2.591 |
| High resolution limit [Å] | 2.502 | 2.502 |
| Rmerge | 0.061 | 0.210 |
| Rmeas | 0.068 | |
| Number of reflections | 8646 | 827 |
| <I/σ(I)> | 17.55 | 6.83 |
| Completeness [%] | 99.8 | 99.52 |
| Redundancy | 5.4 | 4.9 |
| CC(1/2) | 0.996 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 297 | Lithium sulfate, Ammonium sulfate and Sodium citrate (pH 5.6) |
