6ADE
Crystal structure of phosphorylated mutant of glyceraldehyde 3-phosphate dehydrogenase from human placenta at 3.15A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.953 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 90.780, 131.680, 220.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.390 - 3.150 |
R-factor | 0.19627 |
Rwork | 0.194 |
R-free | 0.23937 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u8f |
RMSD bond length | 0.008 |
RMSD bond angle | 1.399 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.390 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.200 | 0.150 |
Number of reflections | 23302 | 1576 |
<I/σ(I)> | 5.6 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 4M sodium formate, pH 7.7 |