6ADE
Crystal structure of phosphorylated mutant of glyceraldehyde 3-phosphate dehydrogenase from human placenta at 3.15A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.953 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.780, 131.680, 220.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.390 - 3.150 |
| R-factor | 0.19627 |
| Rwork | 0.194 |
| R-free | 0.23937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u8f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.399 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.200 | 0.150 |
| Number of reflections | 23302 | 1576 |
| <I/σ(I)> | 5.6 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 4M sodium formate, pH 7.7 |
