6ADA
Crystal structure of the E148D mutant CLC-ec1 in 200mM bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9190 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.409, 99.956, 170.811 |
| Unit cell angles | 90.00, 131.88, 90.00 |
Refinement procedure
| Resolution | 33.969 - 3.153 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ene |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 98122 | 9800 |
| <I/σ(I)> | 18.1 | 2.5 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG400 26%(w/v), 100mM tris-SO4, 200mM NaBr |
