6ACS
poly-cis-prenyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.222, 121.501, 45.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.881 - 1.810 |
| R-factor | 0.1608 |
| Rwork | 0.160 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jp3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.037 | 0.312 |
| Number of reflections | 47890 | 4726 |
| <I/σ(I)> | 33.1 | 4.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 277 | Protein: Tris-HCl, NaCl, glycerol Reservoir: PEG 20000, isopropanol, citric acid Cryoprotectant: glycerol |
