6ACL
histone lysine desuccinylase Sirt5 in complex with succinyl peptide H2AK95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.937, 42.807, 61.591 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 58.150 - 1.920 |
| R-factor | 0.17192 |
| Rwork | 0.169 |
| R-free | 0.22246 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ace |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.150 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.098 | 0.302 |
| Number of reflections | 19522 | 3340 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 92.6 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M sodium cacodylate (pH 5.5), 25% w/v PEG 4000 |
