6ABR
Actin interacting protein 5 (Aip5, wild type)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000,0.9788,0.9636 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.573, 82.775, 34.278 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 32.715 - 2.002 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.26180 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 11332 | 943 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 96.8 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris-HCL pH 7.0, 20% PEG1000 |
