6ABR

Actin interacting protein 5 (Aip5, wild type)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000,0.9788,0.9636
Spacegroup name	P 1 21 1
Unit cell lengths	32.573, 82.775, 34.278
Unit cell angles	90.00, 107.37, 90.00

Refinement procedure

Resolution	32.715 - 2.002
R-factor	0.23
Rwork	0.226
R-free	0.26180
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	11332	943
<I/σ(I)>	19.4
Completeness [%]	96.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris-HCL pH 7.0, 20% PEG1000