6ABO
human XRCC4 and IFFO1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9201 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.315, 331.116, 64.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.640 - 2.650 |
| R-factor | 0.2351 |
| Rwork | 0.231 |
| R-free | 0.27330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ik9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev-3139-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.151 | 0.793 |
| Rmeas | 0.158 | 0.835 |
| Rpim | 0.047 | 0.258 |
| Number of reflections | 24309 | 1207 |
| <I/σ(I)> | 14.61 | 2.91 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.1 | 10.7 |
| CC(1/2) | 0.997 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Sodium cacodylate pH6.0, 50mM Calcium acetate, 20-25% MPD, 1M Ammonium sulfate |
