Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.100, 186.840, 90.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.260 - 1.860 |
| R-factor | 0.19985 |
| Rwork | 0.198 |
| R-free | 0.22691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j49 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.720 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.260 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.097 | 0.827 |
| Rpim | 0.040 | 0.349 |
| Number of reflections | 68606 | 9844 |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 7 | 6.4 |
| CC(1/2) | 0.996 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM sodium acetate (pH 5.5), 8% PEG3350, and 50 mM magnesium chloride. |
