6ABH
Structure of a natural red emitting luciferase from Phrixothrix hirtus (P1 crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-06 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 105.697, 121.174, 129.443 |
Unit cell angles | 61.86, 68.35, 74.17 |
Refinement procedure
Resolution | 48.762 - 3.050 |
R-factor | 0.2408 |
Rwork | 0.240 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6aaa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.622 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.762 | 3.210 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.111 | 1.046 |
Rmeas | 0.123 | 1.163 |
Rpim | 0.053 | 0.497 |
Number of reflections | 98610 | 14365 |
<I/σ(I)> | 14.5 | 2 |
Completeness [%] | 94.1 | |
Redundancy | 5.2 | 5.3 |
CC(1/2) | 0.998 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 19-21% PEG 3350, 0.1M Bis-tris propane (pH 6.0, supplemented with 0.2M ammonium sulfate) |