6AAL
Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with Arginine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.342, 86.000, 123.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.640 - 2.600 |
| R-factor | 0.20509 |
| Rwork | 0.200 |
| R-free | 0.29013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yln |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.618 |
| Data reduction software | HKL-2000 (v703g) |
| Data scaling software | HKL-2000 (v703g) |
| Phasing software | MoRDa (22(2018-05-05)) |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.640 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 15666 | 741 |
| <I/σ(I)> | 9.5 | 1.23 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2M Ammonium sulphate, 0.1M Sodium acetate trihydrate pH 5.5 |
