6AAG
Crystal structure of budding yeast Atg8 complexed with the helical AIM of Hfl1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-02-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.468, 61.650, 100.112 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 45.730 - 2.445 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zpn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.790 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.730 | 2.490 |
| High resolution limit [Å] | 2.445 | 2.450 |
| Rmerge | 0.112 | 0.647 |
| Number of reflections | 40557 | 2002 |
| <I/σ(I)> | 6.5 | 1.1 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 6% PEG6000, 0.1 M sodium citrate pH 5.0 |
