6A9X

Crystal Structure of AnkG/GABARAP Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	I 2 3
Unit cell lengths	96.991, 96.991, 96.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.671 - 2.202
R-factor	0.2048
Rwork	0.203
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kjt
RMSD bond length	0.006
RMSD bond angle	0.933
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.069	0.058
Rmeas	0.071	0.060
Rpim	0.017	0.014	0.335
Number of reflections	7843	420	367
<I/σ(I)>	8.5
Completeness [%]	99.9	98.8	100
Redundancy	19.6	17.5	20
CC(1/2)		0.998	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	289	2.0 M ammonium citrate tribasic, 0.1 M BIS-TRIS propane buffer