6A9C

Crystal Structure c-terminal SH3 domain of Myosin IB from Entamoeba histolytica bound to EhFP10(GEF) peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	77
Detector technology	CCD
Collection date	2015-04-19
Detector	MARRESEARCH
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	29.008, 60.013, 95.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.780 - 1.980
R-factor	0.20307
Rwork	0.201
R-free	0.24009
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgg
RMSD bond length	0.019
RMSD bond angle	1.870
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.780	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.103
Number of reflections	12177	1153
<I/σ(I)>	27.76	3.3
Completeness [%]	100.0
Redundancy	14
CC(1/2)	0.900	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2M Ammonium sulphate, 30% PEG 8000