6A97

Crystal structure of MHC-like MILL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	89.633, 93.497, 138.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.816 - 2.148
R-factor	0.2145
Rwork	0.213
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.148	2.148
Rmeas	0.085	1.313
Rpim	0.032	0.519
Number of reflections	63953	3168
<I/σ(I)>	36.4	1.8
Completeness [%]	99.9	100
Redundancy	7.3	7.3
CC(1/2)		0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M BIS-Tris propane HCl, 20% (w/v) polyethylene glycol 3350, 0.2 M sodium sulfate