6A8W

Crystal structure of the FHA domain of Far9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	31.991, 59.414, 64.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.707 - 1.844
R-factor	0.1937
Rwork	0.191
R-free	0.22270
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.186
Rmeas	0.193
Rpim	0.053	0.403
Number of reflections	11148	550
<I/σ(I)>	19.6	2.4
Completeness [%]	99.6	98.7
Redundancy	13.2	11.1
CC(1/2)		0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	6%(v/v) Tacsimate pH 6.0; 0.1M MES pH 6.0; 25%(w/v) polyethylene glycol 4,000.