6A87
Pholiota squarrosa lectin (PhoSL) in complex with fucose(alpha1-6)GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-05-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.778, 145.778, 39.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.460 - 2.410 |
| R-factor | 0.18028 |
| Rwork | 0.177 |
| R-free | 0.23703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a86 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.486 |
| Number of reflections | 11922 | |
| <I/σ(I)> | 36.7 | 4.4 |
| Completeness [%] | 98.1 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8 M potassium-sodium phosphate, 5% 1,3-butanediol |
