6A86
Pholiota squarrosa lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-05-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.280, 145.280, 39.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.19826 |
| Rwork | 0.196 |
| R-free | 0.24852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xzk |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.391 |
| Number of reflections | 28520 | |
| <I/σ(I)> | 42.2 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.8 M potassium-sodium phosphate (pH 7.0), 5% 1,3-butanediol |
