6A83

Crystal structure of the C-terminal periplasmic domain of EcEptC from Escherichia coli complex with Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 32 2 1
Unit cell lengths	103.568, 103.568, 122.294
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.685 - 2.602
R-factor	0.2136
Rwork	0.212
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a82
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	2.900
High resolution limit [Å]	2.600	2.600
Rmerge	0.125	0.753
Rmeas	0.129	0.772
Rpim	0.029	0.172
Number of reflections	23768	1880
<I/σ(I)>	19.5	4.7
Completeness [%]	100.0	100
Redundancy	19.3
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Ammonium formate (pH7.5), 20% (w/v) PEG3350