6A82
Crystal structure of the C-terminal periplasmic domain of EcEptC from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.060, 85.060, 121.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.830 - 2.100 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.20220 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.089 | 0.427 |
| Rmeas | 0.094 | 0.472 |
| Number of reflections | 56697 | 5219 |
| <I/σ(I)> | 17.6 | 3.1 |
| Completeness [%] | 98.7 | 90.11 |
| Redundancy | 9.5 | 5.48 |
| CC(1/2) | 0.999 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M MES (pH6.5), 15% (w/v) PEG20000 |
