6A7T
Ca2+-independent C-type lectin SPL-1 from Saxidomus purpuratus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.301, 72.602, 45.078 |
| Unit cell angles | 90.00, 95.29, 90.00 |
Refinement procedure
| Resolution | 38.210 - 1.600 |
| R-factor | 0.18582 |
| Rwork | 0.184 |
| R-free | 0.22717 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.565 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.096 | 0.616 |
| Number of reflections | 37306 | |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 293 | 30% PEG3000, 100mM CHES/NaOH (pH 9.5) |
