6A72
Copper transporter protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.114, 87.582, 77.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.790 - 2.100 |
| R-factor | 0.17625 |
| Rwork | 0.174 |
| R-free | 0.21141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cjk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.378 |
| Number of reflections | 9508 | 1670 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 286 | 20%PEG8000,0.1M MES pH6.0,0.2M Ca(oAc)2 |
