6A71
Crystal Structure of Human ATP7B and TM Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.37906 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.267, 87.740, 78.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.870 - 1.600 |
| R-factor | 0.16676 |
| Rwork | 0.165 |
| R-free | 0.19537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cjk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.622 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.292 |
| Number of reflections | 20975 | 3284 |
| <I/σ(I)> | 24.4 | |
| Completeness [%] | 97.8 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 286 | 20%PEG8000,0.1M MES pH6.0,0.2M Ca(OAc)2 |
