6A6R
Crystal structure of the modified fructosyl peptide oxidase from Aspergillus nidulans, Seleno-methionine Derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97980 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 73.199, 73.199, 162.305 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.152 - 2.609 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.25580 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.095 | 0.410 |
Number of reflections | 15887 | |
<I/σ(I)> | 44.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.4 | 293 | 1.75 M ammonium sulfate, 0.2 M lithium sulfate, 0.08 M CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer (pH 10.4) |