6A5N

Crystal structure of Arabidopsis thaliana SUVH6 in complex with methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	74.137, 79.443, 107.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.592 - 2.400
R-factor	0.2332
Rwork	0.230
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a5k
RMSD bond length	0.009
RMSD bond angle	1.314
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.081	0.715
Number of reflections	25062	2497
<I/σ(I)>	32.3	3.4
Completeness [%]	97.9	99.7
Redundancy	5.4	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% ethanol, 10% w/v glycerol