6A5D
Crystal structure of plant Glycosylphosphatidylinositol-anchored Protein LLG1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.212, 66.516, 43.686 |
Unit cell angles | 90.00, 111.13, 90.00 |
Refinement procedure
Resolution | 40.749 - 1.401 |
R-factor | 0.2108 |
Rwork | 0.210 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a5e |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 35300 | 3364 |
<I/σ(I)> | 26.6 | |
Completeness [%] | 95.7 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris pH 6.5, 28% Polyethylene glycol 2,000 |